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Amersham Life Sciences Inc microarray hybridization buffer version 2
Microarray Hybridization Buffer Version 2, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+2+hybridization+buffer/microarray+hybridization+buffer/pm21130852-95-17-23
Average 90 stars, based on 1 article reviews
microarray hybridization buffer version 2 - by Bioz Stars, 2026-09
90/100 stars

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Microarray:

Article Title: A Resource for Transcriptomic Analysis in the Mouse Brain
Article Snippet: Total RNA samples were mixed with 2 μl of SPIKE Mix (GE Healthcare Lucidea universal scorecard), and probes were prepared following the method of , except that 3 μg of cDNA were used per slide, and that the probes were dried and resuspended in 6 μl of water. .. The probes were then denatured at 95°C for 2 min and then mixed with 7.5 μl 4× Microarray hybridization solution version 2 (Amersham, Cat. No RPK0325), 15 μl formamide, and 145 μl of hybridization buffer (2.5 ng/μl oligo(dT) 12–18 primers (Invitrogen), 500 ng/μl mouse Cot-1 DNA (Invitrogen), 0.5× Microarray hybridization buffer version 2 (Amersham, Cat. No RPK0325, stock concentration: 4×), 25% formamide (Nacalai Tesque), 50% ULTRAhyb buffer (Ambion)), at 50°C. .. The mixture of Cy3- and Cy5-labeled probes was applied at 75°C onto arrays, and hybridized for 3 h at 65°C, 3 h at 55°C and 12 h at 50°C in the dark, using an automatic hybridizer, GeneMachines HybStation (Genomic Solutions).

Article Title: Artifactual insulin release from differentiated embryonic stem cells.
Article Snippet: .. Labeled probe corresponding to 17 pmol Cy5 dye was hybridized under coverslips to arrays in 1 Microarray Hybridization Buffer Version 2 (Amersham) that contained 1 mg poly-dA(70) and 50% formamide. .. Arrays were incubated for 16 h at 42°C in a HybChamber (GeneMachine [http://genome.nhgri.nih.gov/genemachine]).

Article Title: Characterization of liver-cirrhosis nodules by analysis of gene-expression profiles and patterns of allelic loss.
Article Snippet: Ten-microgram aliquots of aRNA from normal liver tissues and from nodules or tumor tissues were labeled, respectively, with Cy5-dCTP or Cy3-dCTP (Amersham). .. The labeled probes were mixed with microarray hybridization solution version 2 (Amersham) and formamide (Sigma, St Louis, MO, USA) to a final concentration of 50%. ..

Article Title: Antiviral effect of Phyllanthus nanus ethanolic extract against hepatitis B virus (HBV) by expression microarray analysis.
Article Snippet: Ethanolic extract of Phyllanthus nanus (P. nanus) treatment exhibited potent antiviral activity against Hepatitis B virus (HBV).. The effects of these extracts on HBV in the HBV genome integrated cell lines—Alexander cells and HepG2 2.2.15 cells were examined.. Experimental results showed that the ethanolic extract of P. nanus produced suppressive effect on HBsAg secretion and HBsAg mRNA expression.

Hybridization:

Article Title: A Resource for Transcriptomic Analysis in the Mouse Brain
Article Snippet: Total RNA samples were mixed with 2 μl of SPIKE Mix (GE Healthcare Lucidea universal scorecard), and probes were prepared following the method of , except that 3 μg of cDNA were used per slide, and that the probes were dried and resuspended in 6 μl of water. .. The probes were then denatured at 95°C for 2 min and then mixed with 7.5 μl 4× Microarray hybridization solution version 2 (Amersham, Cat. No RPK0325), 15 μl formamide, and 145 μl of hybridization buffer (2.5 ng/μl oligo(dT) 12–18 primers (Invitrogen), 500 ng/μl mouse Cot-1 DNA (Invitrogen), 0.5× Microarray hybridization buffer version 2 (Amersham, Cat. No RPK0325, stock concentration: 4×), 25% formamide (Nacalai Tesque), 50% ULTRAhyb buffer (Ambion)), at 50°C. .. The mixture of Cy3- and Cy5-labeled probes was applied at 75°C onto arrays, and hybridized for 3 h at 65°C, 3 h at 55°C and 12 h at 50°C in the dark, using an automatic hybridizer, GeneMachines HybStation (Genomic Solutions).

Article Title: Discriminate gene lists derived from cDNA microarray profiles of limited samples permit distinguishing mesenchymal neoplasia ex vivo.
Article Snippet: Background: Mesenchymal neoplasia comprises a heterogeneous group of tumors with over 200 benign neoplasms and 100 sarcomas.. Currently, tumors are classified using histologic and immunocytologic characteristics, with diagnostic error rates reported as high as 40% of cases.. As a feasibility study, our goal was to generate a preliminary discriminatory gene list for selected mesenchymal tumors, including sarcomas.

Article Title: Artifactual insulin release from differentiated embryonic stem cells.
Article Snippet: .. Labeled probe corresponding to 17 pmol Cy5 dye was hybridized under coverslips to arrays in 1 Microarray Hybridization Buffer Version 2 (Amersham) that contained 1 mg poly-dA(70) and 50% formamide. .. Arrays were incubated for 16 h at 42°C in a HybChamber (GeneMachine [http://genome.nhgri.nih.gov/genemachine]).

Article Title: Characterization of liver-cirrhosis nodules by analysis of gene-expression profiles and patterns of allelic loss.
Article Snippet: Ten-microgram aliquots of aRNA from normal liver tissues and from nodules or tumor tissues were labeled, respectively, with Cy5-dCTP or Cy3-dCTP (Amersham). .. The labeled probes were mixed with microarray hybridization solution version 2 (Amersham) and formamide (Sigma, St Louis, MO, USA) to a final concentration of 50%. ..

Article Title: Antiviral effect of Phyllanthus nanus ethanolic extract against hepatitis B virus (HBV) by expression microarray analysis.
Article Snippet: Ethanolic extract of Phyllanthus nanus (P. nanus) treatment exhibited potent antiviral activity against Hepatitis B virus (HBV).. The effects of these extracts on HBV in the HBV genome integrated cell lines—Alexander cells and HepG2 2.2.15 cells were examined.. Experimental results showed that the ethanolic extract of P. nanus produced suppressive effect on HBsAg secretion and HBsAg mRNA expression.

Concentration Assay:

Article Title: A Resource for Transcriptomic Analysis in the Mouse Brain
Article Snippet: Total RNA samples were mixed with 2 μl of SPIKE Mix (GE Healthcare Lucidea universal scorecard), and probes were prepared following the method of , except that 3 μg of cDNA were used per slide, and that the probes were dried and resuspended in 6 μl of water. .. The probes were then denatured at 95°C for 2 min and then mixed with 7.5 μl 4× Microarray hybridization solution version 2 (Amersham, Cat. No RPK0325), 15 μl formamide, and 145 μl of hybridization buffer (2.5 ng/μl oligo(dT) 12–18 primers (Invitrogen), 500 ng/μl mouse Cot-1 DNA (Invitrogen), 0.5× Microarray hybridization buffer version 2 (Amersham, Cat. No RPK0325, stock concentration: 4×), 25% formamide (Nacalai Tesque), 50% ULTRAhyb buffer (Ambion)), at 50°C. .. The mixture of Cy3- and Cy5-labeled probes was applied at 75°C onto arrays, and hybridized for 3 h at 65°C, 3 h at 55°C and 12 h at 50°C in the dark, using an automatic hybridizer, GeneMachines HybStation (Genomic Solutions).

Article Title: Characterization of liver-cirrhosis nodules by analysis of gene-expression profiles and patterns of allelic loss.
Article Snippet: Ten-microgram aliquots of aRNA from normal liver tissues and from nodules or tumor tissues were labeled, respectively, with Cy5-dCTP or Cy3-dCTP (Amersham). .. The labeled probes were mixed with microarray hybridization solution version 2 (Amersham) and formamide (Sigma, St Louis, MO, USA) to a final concentration of 50%. ..

Purification:

Article Title: Discriminate gene lists derived from cDNA microarray profiles of limited samples permit distinguishing mesenchymal neoplasia ex vivo.
Article Snippet: Background: Mesenchymal neoplasia comprises a heterogeneous group of tumors with over 200 benign neoplasms and 100 sarcomas.. Currently, tumors are classified using histologic and immunocytologic characteristics, with diagnostic error rates reported as high as 40% of cases.. As a feasibility study, our goal was to generate a preliminary discriminatory gene list for selected mesenchymal tumors, including sarcomas.

Labeling:

Article Title: Artifactual insulin release from differentiated embryonic stem cells.
Article Snippet: .. Labeled probe corresponding to 17 pmol Cy5 dye was hybridized under coverslips to arrays in 1 Microarray Hybridization Buffer Version 2 (Amersham) that contained 1 mg poly-dA(70) and 50% formamide. .. Arrays were incubated for 16 h at 42°C in a HybChamber (GeneMachine [http://genome.nhgri.nih.gov/genemachine]).

Article Title: Characterization of liver-cirrhosis nodules by analysis of gene-expression profiles and patterns of allelic loss.
Article Snippet: Ten-microgram aliquots of aRNA from normal liver tissues and from nodules or tumor tissues were labeled, respectively, with Cy5-dCTP or Cy3-dCTP (Amersham). .. The labeled probes were mixed with microarray hybridization solution version 2 (Amersham) and formamide (Sigma, St Louis, MO, USA) to a final concentration of 50%. ..

Article Title: Antiviral effect of Phyllanthus nanus ethanolic extract against hepatitis B virus (HBV) by expression microarray analysis.
Article Snippet: Ethanolic extract of Phyllanthus nanus (P. nanus) treatment exhibited potent antiviral activity against Hepatitis B virus (HBV).. The effects of these extracts on HBV in the HBV genome integrated cell lines—Alexander cells and HepG2 2.2.15 cells were examined.. Experimental results showed that the ethanolic extract of P. nanus produced suppressive effect on HBsAg secretion and HBsAg mRNA expression.



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